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Bergey’s Manual of Systematic and Determinative Bacteriology: A Quick Guide: How to use the Manuals

This guide provides students with an overview of how to use the Bergey's Manual of Systematic and Determinative Bacteriology

Helpful Hint

Read the pages titled "on Using the Manual" found at the beginning of each volume

How to use Bergey’s Manual of Systematic Bacteriology

  1. Search Table 2 of the “Road Map” (pp. 142-155) and check to see if the genus of the bacterium is listed.
  2.  If so, see in which volume the information about the bacterium is found.
  3. Then go to the “Index of Scientific names of Archea and Bacteria” at the back of that volume and go to the bolded page number.

Examples:  STREPTOCOCCACEAE, 6, 464, 655,  711

OR

  1. Determine the phylogenetic tree for the bacterium of interest.
  2. Go to the “Contents” pages at the beginning of the volume and look for “Class, Order, Family.”
  3. Under each Family is a list of the Genera that belong to the Family of the bacterium of interest.
  4. It is important to read the material that appears under both the genus and the species.  Information about the genus does not usually repeat on the species description.

Example: PhylumXIII. Firmicutes............................................................19
                              Class II. "Clostridia"..................................................736
                                       Order I. Clostridiales.........................................736
                                                Family I. Clostridiaceae............................736
                                                               Genus I. Clostridium..................738
                                                               Genus II. Alkaliphilus.................828
                                                               Genus III. Anaerobacter.............830

                                                                                        Etc.

How to use Bergey's Manual of Determinative Bacteriology

Step 1. Identify your unknown's group number.

The first thing you'll need to do is determine your unknown's group number. To do that, you will need to consult Bergey's Manual of Determinative Bacteriology (9th edition). To find your group number, look through the table of contents of the manual, and use the table that starts on page 17. Most unknowns will be in one of these groups:

  • Gram-Negative, Aerobic/Microaerophilic Rods and Cocci --Group 4
  • Facultatively Anaerobic Gram-Negative Rods --Group 5
  • Gram-Positive Cocci --Group 17
  • Endospore-Forming Gram-Positive Rods and Cocci --Group 18
  • Regular, Nonsporing Gram-Positive Rods --Group 19
  • Irregular, Nonsporing Gram-Positive Rods --Group 20
  • Aerobic, Nonmotile, Nonsporing, Acid-fast, Weakly Gram-Positive Rods --Group 21

Once you think you know your group number (or you have a few possibilities) go to the pages for your group within the manual. From there, you should find more information to help you make a final determination that you have the right group. You might also consult the information below to help you find the best tables to make a final determination about your unknown's group number:

Group #

Table to reference in Bergey’s Determinative, 9th edition

Key differences between genera in this group, as described in the Bio 205L manual

4

Table 4.1, pp. 103-116

pigments/fluorescent, motility, growth requirements, denitrification, morphology, oxidase

5

Table 5.1, pg. 202

growth factors, morphology, gram reaction, oxidase

17

Aerobic genera: Table 17.1, pg 534

 

Facultatively anaerobic genera: Table 17.2, pg. 535-536

 

Strictly anaerobic genera: Table 17.3, pg. 537

oxygen requirements, morphology, growth requirements (45°C and supplements)

18

Table 18.1, pg. 562

oxygen requirements, motility, morphology, catalase

19

Table 19.1, pg. 568

morphology, oxygen requirements, catalase

20

Aerobic genera: Table 20.1, pg. 583-584

 

Facultatively anaerobic genera: Table 20.2, pg. 585-586

 

Strictly anaerobic genera: Table 20.3, pg. 587-588

catalase, motility, morphology

21

Table 21.1, pg 598

 

acid fast, growth

 

Step 2: Determine the genus of your unknown.

To determine the genus of your unknown, you'll need to keep using Bergey's Manual of Determinative Bacteriology (9th edition). You should have the right group number by now, so go to the pages for your group. These pages should help you identify the genus of your unknown. There might be both tables and descriptions to help you identify the genus. You might find more than one possible match -- that's ok. The next step should provide more information to help you narrow down to a final choice.

Step 3. Read about your genus to make sure you have a match.

Step 4. Identify your unknown to the species level.

The genus description should contain information that helps you differentiate the species in your genus – so, you can compare your lab results to attempt to identify the species of your unknown. Be sure to read both the genus and the species descriptions, because characteristics listed in the genus description aren’t repeated in the species description!

Step 5. Troubleshoot problems.

If you find that the tables in Bergey's Manual of Determinative Bacteriology, 9th edition only allowed you to identify your bacteria down to the family level, then search the Systematic manual (link provided above) for your family name to see if the family description contains the tables you need to narrow down from family to genus. If the Systematic manual DOESN’T contain the tables you need, then you’ll have to come to the Main Desk at Cline Library and to once again consult Bergey's Manual of Determinative Bacteriology, 9th edition. Look up your family name in the index of the Determinative manual to see if there are tables you need that you previously missed.

Also keep in mind that sometimes a taxonomic name can be declared a synonym of another name, and thus is no longer used. Try looking your name up in either of these two resources: 

If you find that another name is being used, look that name up in Bergey's instead. For example, the genus name Aurobacterium has been synonomized with Microbacterium, so you'd look up Microbacteriuminstead

Adapted from Nothern Arizona University LibGuide URL: https://libraryguides.nau.edu/bio205l-305w

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